TABLE 2.
Summary of polymorphic sites within the ITS1, 58S, and ITS2 regions inT. dubius,T. porrifolius, andT. pratensis
| ITS1
|
5.8S gene: | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Species | −244 | −223 | −201 | −188 | −183 | −150 | −123 | −52 | −21 | −16 | −6 | +122 | |
| T. dubius | A | A | G | C | T | G | T | T | — | C | C | C | |
| T. pratensis | A | A | A | C | T | G | T | C | G | T | G | T | |
| T. porrifolius | G | G | G | T | T | A | C | C | G | T | G | C | |
| T. mirus 2602 “novel” family | A/g (60%) | A/g (80%) | G/a (60%) | C/t (60%) | T/c (80%) | G | T/c (80%) | C | G | T | G | ND | |
| |||||||||||||
| ITS2
| |||||||||||||
| Species | +174 | +175 | +177 | +193 | +199 | +210 | +212 | +232 | +241 | +242 | +243 | +274 | +317 |
| T. dubius | C | C | C | G | G | G | C | T | C | T | C | T/A | C |
| T. pratensis | — | — | T | G | A | T | C | C | C | C | T | T | C |
| T. porrifolius | — | — | T | A | G | G | T | C | T | T | T | T | T |
Positions of nucleotides are numbered with respect to the first nucleotide of the 5.8S gene [+1 (A) was taken from aligned Arabidopsis sequences in the database]. The recombinant character of the unusual T. mirus 2602 family is indicated by shaded rectangle. The frequency of a dominant nucleotide (in uppercase letters) at variable positions is indicated. —, gap; ND, not determined.