Figure 6.—

A diagrammatic representation of the cytogenetic locations of 19 sorghum BACs from sorghum LG-08 and the corresponding marker positions (Menz et al. 2002). (A) Linkage group 8 markers used to select BACs for FISH (Menz et al. 2002). (B) Diagram of the pachytene bivalent indicating the positions that contain LG-08 marker loci. Green circles, FITC detection; red boxes, Cy3 detection. (C) Estimation of the strength and location of BAC probes for Figure 5. Graph peaks represent relative strengths of fluorescence signals and their distributions; blue represents DAPI signal from chromosomal DNA, green represents FITC signal, and red represents Cy3 signal. Arrowheads indicate saturated signal from DAPI and arrow indicates signals of DAPI from overlapping strands of the pachytene bivalents.