TABLE 2.
Conversionsc
|
|||||||
---|---|---|---|---|---|---|---|
Mutation | Positiona | Sequence change | Cross typeb |
6:2 | 2:6 | Tetrads analyzed |
Conversion (%) |
13 | 562 | C to A | II | 24 | 13 | 208 | 17.8 |
1 | 634 | A to G | I | 13 | 24 | 213 | 17.4 |
5 | 709 | G to T | I | 6 | 20 | 214 | 12.1 |
6 | 793 | G to C | I | 10 | 7 | 200 | 8.5 |
14 | 823 | C to A | I | 8 | 16 | 217 | 11.1 |
9 | 934 | G deletion | II | 14 | 5 | 216 | 8.8 |
12 | 1001 | G insertion | I | 4 | 15 | 217 | 8.8 |
2 | 1043 | G to A | I | 8 | 8 | 211 | 7.6 |
4 | 1066 | G to A | II | 4 | 8 | 153 | 7.8 |
3 | 1104 | C to T | I | 15 | 12 | 220 | 12.3 |
11 | 1163 | 22-bp repeat | II | 13 | 2 | 205 | 7.3 |
10 | 1167 | C to T | I | 2 | 10 | 204 | 5.9 |
Numbering according to Grimm et al. (1988). First base of start codon is 534; first base of stop codon is 1326.
Cross type I is h− ura4A− × h+ ura4A+; cross type II is h− ura4A+ × h+ ura4A−.
In this tetrad run it was not possible to detect PMS. A conversion of 6:2 means three ura4A+ spore colonies (prototrophs) and one ura4A− colony (auxotroph); 2:6 conversion tetrads have only one ura4A+ spore colony.