Table 1.
Primer sequences and RT-PCR amplification conditions.
| Gene | Primer sequence | Annealing temperature, °C | No. of cycles | Product size, bp |
|---|---|---|---|---|
| COL-I | 5′-TTGACCCTAACCAAGGATGC
5′-CACCCCTTCTGCGTTGTATT |
54 | 30 | 197 |
| COL-III | 5′-AGGCCAATGGCAATGTAAAG
5′-GGCCTTGCGTGTTTGATATT |
53 | 30 | 187 |
| TGF-β1 | 5′-CACCTGCACAGCTCCAGGCAC
5′-CTTGCGACCCACGTAGTAGACG |
54 | 32 | 153 |
| HGF | 5′-TCGTTCCTTGGGATTATTGC
5′-TGGTGCTGACTGCATTTCTC |
55 | 32 | 571 |
| TIMP-1 | 5′-TCCCCAGAAATCATCGAGAC
5′-ATGGCTGAACAGGGAAACAC |
55 | 32 | 329 |
| LH2b | 5′-TGCGATCAGAGATGAGTGAA
5′-GTTGAGATGGGAAGTGTTGTAAT |
53 | 32 | 229 |
| E-cadherin | 5′-GCAGTTCTGCCAGAGAAACC
5′- AATCCTGCTTCCAGGGAGAT |
55 | 28 | 315 |
| N-cadherin | 5′-TGTTGCTGCAGAAAACCAAG
5′-GGCGACTCTCTGTCCAGAAC |
53 | 28 | 309 |
| DSG-2 | 5′-CCTTGGTGGACCAGCACTAT
5′-GACAGCACCGTCTTCCTAGC |
57 | 35 | 316 |
| DSC-2 | 5′-CGTAATTCCCGTCCGAGTTA
5′-TTGAGCTGTGAAGCCATTTG |
55 | 35 | 386 |
| GAPDH | 5′-ATGGTGAAGGTCGGTGTGAAC
5′- GCTGACAATCTTGAGGGAGT |
54 | 28 | 473 |