Estimated initial frequency, relative to TM1/+ viability, compared between replicates: p0/VTM1/+. Large circles, “invader” lines; small circles, “transient” lines; solid circles, +/+ lethal; dark-shaded circles, +/+ lethal from Fowler et al. (1997); light-shaded circles, +/+ viable. One estimate from transient +/+ viable lines lies off scale at bottom left (chromosome 32: A, 3.7 × 10−24; B, 1.1 × 10−20).