TABLE 5.
Non-Mendelian segregation ofhis4-ATC andBIK1-939
| NMS class
|
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Allele | Relevant genotype | 6:2 | 2:6 | 5:3 | 3:5 | 8:0 | 0:8 | 7:1 | 1:7 | Ab4:4 | Othersa | Total NMS |
Total tetrads |
NMS (%) |
FC/NMS (%) |
| his4-ATC | Wild type | 96 | 111 | 14 | 15 | 1 | 3 | 1 | 2 | 0 | 0 | 243 | 1731 | 14 | 87 |
| mlh1Δ | 5 | 7 | 35 | 65 | 0 | 0 | 0 | 0 | 2 | 2 | 116 | 585 | 20b | 12 | |
| msh2Δ | 15 | 18 | 17 | 36 | 0 | 1 | 0 | 1 | 3 | 3 | 96 | 545 | 18 | 37 | |
| Wild-type subsetc | 1 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 107 | 9.3 | 80 | |
| mlh1Δ subsetc | 2 | 0 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 22 | 106 | 21 | 9 | |
| msh2Δ subsetc | 2 | 3 | 3 | 11 | 0 | 0 | 0 | 0 | 1 | 1 | 21 | 101 | 21 | 24 | |
| BIK1-939 | Wild-type subsetc | 0 | 9 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 10 | 107 | 9.3 | 100 |
| mlh1Δ subsetc | 0 | 1 | 6 | 4 | 0 | 2 | 0 | 0 | 1 | 0 | 14 | 106 | 13 | 21 | |
| msh2Δ subsetc | 0 | 0 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 101 | 8.9 | 0 | |
| Wild type selectedd | 38 | 51 | 2 | 1 | 1 | 6 | 0 | 1 | 0 | 0 | 100 | 144 | 69 | 96 | |
| mlh1Δ selectedd | 0 | 3 | 23 | 25 | 1 | 0 | 0 | 0 | 2 | 1 | 51 | 116 | 44b | 10 | |
| msh2Δ selectedd | 0 | 4 | 14 | 23 | 0 | 0 | 0 | 0 | 2 | 0 | 43 | 96 | 45b | 10 | |
FC, full conversion.
a
Mainly Ab6:2 and Ab2:6.
b
Statistically significant compared to the equivalent wild-type proportion (P < 0.025; G-test for homogeneity).
c
Randomly chosen tetrads were analyzed by PCR to estimate the NMS frequencies of BIK1-939 and HIS4-1605.
d
Tetrads that were selected for an NMS at his4-ATC and were analyzed further for NMS at BIK1-939 and HIS4-1605.