Figure 2.—

Isolation of doh alleles. (A) Location of the transposition insertion alleles that disrupt the ORFs of CDC25 and RAM1 and exhibit a doh phenotype. (B) The wild-type reporter strain (YHN802), the reporter strain harboring the doh8 ram1::Tn7 transposition allele (YHN941), and the reporter strain harboring the doh12 cdc25::Tn7 transposition allele (YHN947) were struck on nonselective SC Dex −Ura medium or selective SC Dex −Ura −His medium containing 30 mm 3-AT. (C) The wild-type reporter strain (YHN802), the reporter strain with a ram1Δ allele (YHN1126), and the reporter strain with a cdc25Δ allele (YHN1128) were struck on nonselective SC Dex −Ura medium or selective SC Dex −Ura −His medium containing 30 mm 3-AT.