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MSN2 and MSN4 are not required for doh phenotypes. (A) The msn2/4Δ reporter strain (YHN966), the msn2/4Δ reporter strain with the ram1Δ allele (YHN1077), and the msn2/4Δ reporter strain with the cdc25Δ allele (YHN1078) were struck on nonselective SC Dex −Ura medium or selective SC Dex −Ura −His medium containing 30 mm 3-AT. (B) A Northern blot of total RNA isolated from the indicated strains grown at 30° was probed with a HSP26-specific probe, with ACT1 as a loading control.
