Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Mar;169(3):1203–1214. doi: 10.1534/genetics.104.034256

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, Genetics Society of America

PMC Copyright notice

Figure 8.— — Deletion of PKA increases Hsf1p phosphorylation. The phosphorylation state of Myc-tagged Hsf1 was analyzed in strains YHN1172 (HSF1-13Myc::TRP1 msn2/4Δ) and YHN1173 (HSF1-13Myc::TRP1 msn2/4Δ tpk1/2/3Δ). Yeast expressing Myc-tagged Hsf1 were grown at 30° (heat shock −) or heat-shocked at 40° for 30 min (heat shock +). Hsf1-13Myc was enriched by immunoprecipitation from cell extracts and split into two pools. These samples were incubated with and without calf intestinal alkaline phosphatase (CIP +/−) and resolved on a 7.5% SDS-PAGE, and Hsf1-13Myc was detected by Western blotting with Myc antibodies.