Figure 7.—

The effect of the internal ΔE deletion on Msn2 degradation. The yeast strains MZ161-8A (msn2Δ; A) and MZ161-41 (msn2Δsrb10Δ; B) were each transformed with YCp(33)MSN2ΔE. In addition, MZ161-8A was transformed with YCplac33 (for lanes marked C) or YCp(33)MSN2HA (for lanes marked WT). Cell cultures were grown on SC minus uracil medium to midexponential phase, and cell extracts were prepared for immunoblots as described in materials and methods. Each immunoblot was probed sequentially with anti-HA and anti-eIF5A antisera. The positions of the full-length Msn2HA and the ΔE deletion mutant are shown to the right of the blot.