Figure 1.—
Construct for RNAi of lolC genes. (A) Map of the plasmid construct. (B) Map of the lolC sequences in the construct. Open and shaded boxes are, respectively, the 5′-UTR and coding sequences in exon 1. (C) Sequence of the lolC first intron (lowercase letters) between the complementary first exon sequences (uppercase letters). Underlined sequence indicates the putative intron branch point, and italicized sequence indicates the XbaI site introduced for cloning.