Figure 5.—

(A) Expression of lolC and (B) loline-alkaloid production in N. uncinatum transformed with pKAES178 or empty vector (vector-only). The graphs show the means of five independent experiments; expression of the housekeeping gene, tub2, in the lolC-RNAi and vector-only transformants is indicated in A. Loline-alkaloid production (based on total lolines per gram fungal DW) is expressed as percentage of the mean in the vector-only controls in each experiment. Error bars indicate the standard error of the mean.