Figure 2.—

DNA replication is attenuated after mus-101 RNAi by soaking. (A) Wild-type or mus-101 soaking RNAi embryos were fixed, treated with RNase I, and stained with propidium iodide to visualize the DNA (“DNA”) and with anti-α-tubulin to visualize tubulin (“tubulin”). (B) Schematic of the uncoupling of DNA replication from cell division that occurs upon treatment of permeabilized embryos with the cytokinesis inhibitor cytochlasin B. (C) Permeabilized embryos were fixed and stained with Hoechst's 33258 stain either immediately after permeabilization (I) or after a 1-hr incubation in culture media (II–IV). The embryos shown in II and IV are wild type, while the embryo depicted in III was depleted of mus-101 gene product by soaking RNAi. The embryo in IV was treated with HU to block replication. The embryos depicted in I–IV are representative of a larger (>25) sample set that was analyzed for each condition.