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Screening strategy for isolation of mus-101 modifiers by dual RNAi. Well A is the control plate that contains bacteria expressing dsRNA against the gene to be screened with dsRNA against an irrelevant protein (GFP). This produces N% embryonic lethality (emb). When dual RNAi is performed against the gene to be screened and mus-101 (well B), then the percentage of emb must be >10 and at least twice N for the gene to be pursued further.
