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The LF1 gene contains eight exons and requires only the amino-terminal half of the protein for rescue of the lf1 phenotype. Shown is a restriction map of the 7.2-kb genomic plasmid (p7.2BB) containing the LF1 gene with the exons indicated as solid bars. Various subclones were able to rescue the lf1 phenotype upon transformation including three (p4.6BN, p3.9NN, and p5NN) that lack the 3′ end of the LF1 gene. Arrow indicates location of lf1 mutation.
