Figure 2.—

The integrated recombination and physical maps for recombinant A. roylei × A. fistulosum (RF), chromosome 5. The recombination and physical maps of the recombinant RF chromosome are split into the recombination and physical maps of A. roylei (left) and the recombination and physical maps of A. fistulosum (right). Horizontal lines on the physical maps indicate sites of recombination between A. roylei and A. fistulosum dividing the chromosome into subregions. The genotype numbers, from which the correspondent recombination sites were obtained, are shown in boldface type. On the recombination maps, markers are indicated as follows: e35m52a-302f means restriction enzyme combination EcoI/MseI, primers e35 and m52; “a” identifies the additional seventh selective base, 302 is the length of the fragment, and “f” or “r” specifies whether a marker is specific for A. roylei or for A. fistulosum. Recombination maps and physical maps are linked to each other by lines connecting observed physical recombination sites and their corresponding genetic sites.