TABLE 5.
Parental genotypea |
Ade+ recombinants/106 viable sporesb |
|||
---|---|---|---|---|
Cross series | rad16 | rec12 | SI × 52 | M-pal × 52 |
15 | + | + | 150 ± 7 | 1150 ± 57 |
16 | Δc | + | 140 ± 11 | 440 ± 32 |
17 | + | Δ152 | 2 ± 0.3 | 405 ± 89 |
18 | Δ | Δ152 | 6 ± 1 | 100 ± 19 |
Strains with either ade6-3034 (single insertion; SI) or ade6-3036 (M-pal) were crossed with strain GP4655 [relevant genotype: h−(smt-0) ade6-52 rec12Δ rad16Δ]. Crosses were homozygous for the indicated mutations and heterozygous for the indicated wild-type (+) alleles. Crosses were performed at 25°.
Values are the means of three independent experiments ±SEM.
The rad16::ura4+ (Δ) is a deletion of the rad16 ORF (Carr et al. 1994).