Skip to main content
. 2005 Mar;169(3):1261–1274. doi: 10.1534/genetics.104.037515

TABLE 5.

Rad16 stimulates specifically M-pal-dependent recombination

Parental
genotypea
Ade+ recombinants/106
viable sporesb
Cross series rad16 rec12 SI × 52 M-pal × 52
15 + + 150 ± 7 1150 ± 57
16 Δc + 140 ± 11  440 ± 32
17 + Δ152   2 ± 0.3  405 ± 89
18 Δ Δ152   6 ± 1  100 ± 19
a

Strains with either ade6-3034 (single insertion; SI) or ade6-3036 (M-pal) were crossed with strain GP4655 [relevant genotype: h(smt-0) ade6-52 rec12Δ rad16Δ]. Crosses were homozygous for the indicated mutations and heterozygous for the indicated wild-type (+) alleles. Crosses were performed at 25°.

b

Values are the means of three independent experiments ±SEM.

c

The rad16::ura4+ (Δ) is a deletion of the rad16 ORF (Carr et al. 1994).