TABLE 1.
RNAi analysis of duplicatedrpl genes in wild-type (N2) andrrf-1(pk1417) mutant animals
| Phenotypea
|
|||||||
|---|---|---|---|---|---|---|---|
| Germ-line defect
|
Somatic defect
|
||||||
| RNAi target | LG | Strain | WTb (%) | Sterilec (%) | Unhealthyd (%) | Gro/Lvae (%) | n |
| (None) | N2 | 76 | 0 | 21 | 0.7 | 275 | |
| rrf-1 | 24 | 25f | 40 | 0 | 114 | ||
| rpl-11.1 | V | N2 | 0 | 77f | 5 | 0.7 | 298 |
| rrf-1 | 0 | 75f,g | 5 | 0 | 59 | ||
| rpl-11.2 | X | N2 | 3 | 12 | 3 | 78 | 295 |
| rrf-1 | 0 | 15g | 45 | 10 | 80 | ||
| rpl-25.1 | X | N2 | 73 | 0 | 25 | 0 | 149 |
| rrf-1 | 44 | 43f,g | 7 | 4 | 99 | ||
| rpl-25.2 | I | N2 | 8 | 86f | 6 | 0 | 152 |
| rrf-1 | 10 | 68f,g | 5 | 0 | 19 | ||
| rpl-24.1 | I | N2 | 1 | 22f,g | 15 | 62 | 112 |
| rrf-1 | 0 | 76f | 17 | 0.2 | 58 | ||
| rpl-24.2 | I | N2 | 24 | 10g | 18 | 47 | 180 |
| rrf-1 | 0 | 89f | 11 | 5 | 121 | ||
Data are reported here for one parallel experiment; several experiments gave similar results (see materials and methods). Percentages for phenotypes that were most penetrant for each treatment are in italic type. Remaining animals fell into “other” phenotypic categories that were not scoreable, such as lethal (Let) or ruptured (Rup).
Normal-sized fertile adults.
Sterile adults (both normal sized and reduced size).
Fertile adults but small or sick looking.
Larval arrest (Lva) or larval-sized animals.
Majority of sterility due to strong Glp (germ-line proliferation defective) phenotype; this phenotype is observed variably in the rrf-1 background and may be temperature sensitive. In the particular experiment reported here, the penetrance is on the high side of the range of variability compared to other experiments.
Sterility largely due to mild germ-line proliferation defect often accompanied by signs of gametogenesis (f,g indicates that a large number of worms exhibited both types of sterility defect).