Figure 1.—

Genetic cross and resulting progeny to generate a segregating population for mle and mof. The cross +/Y; mle[pml8]/SM6a × y[1] mof[1]/FM7(GFP); mle[pml8]/mle[pml8] produces male progeny that are segregating for both mle and mof. The four types of males are normal [FM7(GFP)], mutant for mle alone (mle[pml8]/mle[pml8]), mutant for mof alone (mof[1]), and mutant for both mof and mle (mof[1]; mle[pml8]/mle[pml8]). The presence or absence of GFP, SXL, and MLE can be used to distinguish all four classes of males.