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. 2006 Mar 1;103(11):3978–3982. doi: 10.1073/pnas.0509372103

Fig. 1.

Fig. 1.

Aggregation and cleavage experiments. (A) Color of the gold colloids. (a) untreated solution. (b) Five minutes after the addition of 1 ([1] = 62 nM). (c) Five minutes after the addition of 1 ([1]final = 62 nM) incubated for 90 min with thrombin ([thrombin] = 35 nM, [1] = 62 μM). (B) RP-HPLC chromatogram of the original peptide 1 (trace a) and after exposition for 60 min to thrombin (trace b). Conditions: [1]final = 62 μM, [thrombin] = 30 nM, pH 8, 25°C. The peak at 21.5 min corresponds to the fragment Ac-Cys(S-Ac)-Gly-dPhe-Pro-Arg-OH.