Fig. 2.

SB transposase down-regulates cyclin D1 expression. Differential expression of selected cell-cycle regulatory genes in human HeLa cells in response to the presence of the SB transposase. Shown are relative changes of transcript levels in transgenic cells stably expressing the SB transposase compared with wild-type cells, in the presence and absence of a plasmid containing a GFP-tagged transposon (pT/GFP). Cyclin D1 protein levels are reduced in IRES-SB cells, as revealed by Western blot hybridization.