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. 2006 Mar 6;103(11):4068–4073. doi: 10.1073/pnas.0508295103

Fig. 3.

Fig. 3.

hTOPO IIIα can be replaced by bacterial type IA topoisomerases in dissolution reactions. (A) Dissolution reactions containing BLM (5 nM) and hTOPO IIIα (250 nM), E. coli Top1 (50 nM), E. coli Top3 (15 nM), or wheat germ topoisomerase I (10 units), as indicated above the lanes. The positions of DHJ and the detectable dissolution product are indicated on the left. (B Upper) Dissolution reactions containing BLM (5 nM), as indicated by the black bars, hRMI1 (166 nM), as indicated by the white bar, and a 2-fold dilution series of E. coli Top1 (highest concentration of 8 nM), as indicated by the gray triangles. The positions of DHJ and the detectable dissolution product are indicated on the left. (B Lower) Quantification of the dissolution product in lanes 2–6 (−hRMI1) and lanes 9–13 (+hRMI1) as a function of E. coli Top1 concentration. (C Upper) Dissolution reactions containing BLM (5 nM), as indicated by the black bars, hRMI1 (166 nM), as indicated by the white bar, and a 2-fold dilution series of E. coli Top3 (highest concentration of 11 nM), as indicated by the gray triangles. The positions of DHJ and the detectable dissolution product are indicated on the left. (C Lower) Quantification of the dissolution product in lanes 2–6 (−hRMI1) and lanes 9–13 (+hRMI1) as a function of Top3 concentration.