Table 1.

Kinetic data for S6 wild-type and the circular permutants

Mutations	log ku	mu, M−1	log kf	mf, M−1	mD-N,* M−1	β‡	Midpoint	ΔGD-N, kcal/mol	Relative contact order, %
S6wt	−3.73	0.59	2.53	−1.21	1.80	0.67	3.47	8.51	18.9
P13–14	−0.14	0.64	2.85	−1.01	1.65	0.61	1.81	4.07	12.8
P54–55	−3.36	0.68	3.26	−0.93	1.61	0.58	4.10	9.00	19.7
P68–69	−2.45	0.62	2.72	−1.28	1.90	0.67	2.72	7.04	14.3

*m_D-N = m_u − m_f.