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. 2006 Feb 27;103(11):4083–4088. doi: 10.1073/pnas.0508863103

Table 2.

φ-values and stabilities for S6 wild-type and the circular permutants

Mutations φ S6wt* ΔΔG§ S6wt mu S6wt p13–14 φ P13–14† ΔΔG§P13–14 muP13–14 P54–55 φ P54–55 ΔΔG§ P54–55 mu P54–55 P68–69 φ P68–69∥ ΔΔG§ P68–69 mu P68–69
WT 0.59 ± 0.04 0.64 ± 0.04 0.68 ± 0.02 0.62 ± 0.02
V6A 0.52 3.74 0.40 ± 0.01 V89A 0.92 3.01 0.72 ± 0.06 V48A 0.57 3.45 0.63 ± 0.02 V34A 0.50 3.12 0.72 ± 0.02
I8A 0.46 4.24 0.51 ± 0.01 I91A 0.58 3.40 0.79 ± 0.12 I50A 0.38 3.52 0.72 ± 0.02 I36A 0.42 3.14 0.79 ± 0.02
L19A 0.24 2.70 0.50 ± 0.01 L7A 0.09 0.59 1.29 ± 0.10 L61A 0.03 1.90 0.67 ± 0.01 L47A 0.23 1.65 0.60 ± 0.02
I26A 0.40 3.13 0.65 ± 0.01 I14V 0.15 0.98 0.94 ± 0.15 I68V 0.16 1.20 0.85 ± 0.03 I54V 0.39 1.27 0.60 ± 0.03
L30A 0.34 3.74 0.78 ± 0.03 L18A 0.09 2.63 0.95 ± 0.16 L72A 0.13 2.84 0.61 ± 0.03 L58A 0.36 2.84 0.62 ± 0.04
V37A 0.24 2.74 0.51 ± 0.02 V25A 0.05 1.87 0.64 ± 0.07 V79A −0.05 1.14 0.50 ± 0.02 V65A 0.13 1.56 0.61 ± 0.03
V40A — 0.76 0.59 ± 0.03 V28A — 0.19 0.80 ± 0.08 V82A — 0.10 0.70 ± 0.02 V68A — 0.26 0.61 ± 0.01
L48A — 0.74 0.49 ± 0.03 L36A — 0.19 0.71 ± 0.02 L90A — 0.40 0.59 ± 0.01 L76A — 0.13 0.56 ± 0.02
F60A — 1.32 0.44 ± 0.03 F48A — 0.70 0.56 ± 0.02 F7A — 0.68 0.63 ± 0.02 F88A — 0.58 0.56 ± 0.02
L61A 0.24 3.52 0.54 ± 0.01 n.d. n.d. n.d. n.d. L8A 0.12 3.00 0.59 ± 0.02 L89A 0.17 3.23 0.46 ± 0.02
Y63A 0.21 4.00 0.50 ± 0.01 Y51A 0.17 1.74 0.83 ± 0.04 Y10A 0.05 2.67 0.58 ± 0.03 Y91A 0.21 2.86 0.57 ± 0.02
V65A 0.38 3.42 0.58 ± 0.02 V53A 0.26 2.49 0.74 ± 0.05 V12A 0.15 2.79 0.54 ± 0.02 V93A 0.30 2.76 0.60 ± 0.05
V72A 0.14 1.48 0.52 ± 0.02 V60A 0.25 0.86 0.51 ± 0.04 V19A 0.10 1.04 0.68 ± 0.03 V5A 0.16 0.40 0.72 ± 0.05
L75A 0.40 2.17 0.50 ± 0.03 L63A 1.51 1.19 0.61 ± 0.02 L22A 0.54 1.93 0.63 ± 0.04 L8A 0.17 1.79 0.77 ± 0.03
L79A 0.16 4.84 0.53 ± 0.02 n.d. n.d. n.d. n.d. L26A 0.21 4.16 0.78 ± 0.02 L12A 0.12 3.64 0.75 ± 0.02
V85A 0.07 3.55 0.48 ± 0.01 V73A 0.42 1.41 0.61 ± 0.04 V32A 0.15 2.69 0.73 ± 0.02 V18A 0.14 2.72 0.69 ± 0.03
V88A 0.14 2.13 0.39 ± 0.02 V76A 1.10 1.26 0.64 ± 0.02 V35A 0.57 1.57 0.75 ± 0.03 V21A 0.35 1.53 0.66 ± 0.02
V90A 0.14 2.99 0.33 ± 0.02 V78A 0.70 2.18 0.75 ± 0.04 V37A 0.56 2.39 0.65 ± 0.03 V23A 0.38 2.01 0.64 ± 0.03

The columns indicate the translation of sequence positions between wild-type and permutant proteins, even though the mutations are numbered according to the wild-type sequence throughout the text. Data points corresponding to rates faster than 200 s−1 were excluded from the fits. n.d., not determined.

*Calculated from Eq. 1 with A = 1.0 M and B = 4.0 M (13).

†Calculated from Eq. 1 with A = 0.5 M and B = 3.0 M.

§ΔΔGD-N (kcal/mol) was calculated as mD-N × [GdmCl]50% × 2.3 RT.

Data fitted in the interval 0–5.5 M GdmCl. Calculated from Eq. 1 with A = 1.0 M and B = 5.0 M.

∥Data fitted in the interval 0–5 M GdmCl. Calculated from Eq. 1 with A = 1.0 M and B = 5.0 M.