Fig. 5.

Overexpression of CENP-A correlates with reduction of H3K9me2 at human centromeres. ChIP-PCR of histone modifications at three human centromeres (D7Z1, D17Z1, and DXZ1) in CENP-A-overexpressing cell lines showed increased CENP-A enrichment at α-satellite from chromosomes 7 and X and concurrent decrease in H3K9me2. Depending on the centromere, H3K9me3 increased. % IP for histone modifications at α-satellite arrays was compared as relative ratios of each modification. Bar graphs are relative average ratios (% IP over input DNA) with standard deviations.