Fig. 2.

TBK1 induces expression of secreted factors. The induction of RANTES and IL8 in recombinant TBK1-expressing cells was analyzed by qPCR using as reference the housekeeper G6PDH, which was tested in parallel in those experiments. (A) RNA of TBK1-transfected HEK293 cells was extracted at different time points after transfection, and mRNA levels were determined by qPCR. The relative signals (normalized to housekeeper expression) of the PCR reactions of RANTES and IL8 are different (RANTES, left axis; IL8, right axis), but both factors are induced with similar kinetics. (B) RANTES and IL8 induction by supernatants of transiently transfected cells is also observed in the MCF7 breast cancer cell line. Induction of the cytokines was detected as described in A.