Fig. 4.

B2 does not alter chaperone levels or activity. (A) Western blot for levels of chaperones in H4 cells transfected with vector or α-synuclein and treated with B2 or DMSO. Hsp70, Hsp27, and DJ-1 are not significantly altered by B2 treatment. (B) Refolding of heat-denatured firefly luciferase protein in rabbit reticulocyte lysate, in the presence of DMSO, B2, or Hsp90 inhibitor geldanamycin (GA). GA decreases the rate of refolding by >50% in this assay, whereas DMSO and B2 have no effect. Values are the mean of two assays, and error bars show SD.