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A53T, like WT, principally localizes to the plasma membrane, while A30P remains in the cytoplasm. (A) Y382 cells expressing WT, A30P, and A53T α-Syn were grown to midlog phase, galactose induced, and photographed as in Figure 1. (B) As in A; images were taken after two doublings (∼4 hr). White arrow, α-Syn localization at the bud neck; black arrows, α-Syn localization within the nucleus.
