Figure 4.—

Intracellular localization of A53T, but not that of A30P, is disrupted in sec mutants. (A) Localization of GFP and the indicated GFP-α-Syn fusion proteins in the sec9 mutant LRB934 at either 23° or 37° was determined by fluorescence and differential interference microscopy. Cultures were pregrown in 2% raffinose −Ura medium to early log, and then galactose was added to a final concentration of 2% simultaneous with temperature upshift (37° samples). Cells were harvested and photographed 2 hr later. (B) As in A, except the sec14 mutant LRB933 was employed.