Figure 6.—

WT and A30P form heterogeneous-sized aggregates under conditions of overproduction. WCEs isolated from Y382 cells (pregrown in 2% sucrose −Leu medium; galactose induced for 4 hr) were fractionated on a Sephacryl S-300HR column. Protein from 1-ml fractions was precipitated with 10% trichloroacetic acid. The precipitate was washed with acetone, resolubilized in 20 μl of SDS-polyacrylamide gel electrophoresis loading buffer, and subjected to immunoblot analysis. The column was calibrated using blue dextran (2 MDa), thyroglobulin (669 kDa), apoferritin (440 kDa), catalase (232 kDa), aldolase (158 kDa), ovalbumin (44 kDa), chymotrypsinogen A (25 kDa), and myoglobin (17 kDa).