Figure 8.—

α-Syn induces the heat-shock response. (A) Induction of hsp82-lacZ in response to thermal and oxidative stress. Strain EVS1013 was maintained at 30°, subjected to a heat shock (HS), or exposed to 0.3, 0.6, or 1.2 mm hydroperoxide for the times indicated. Cells were harvested and lysed, and extracts were assayed for β-galactosidase activity (expressed in Miller units). For control, heat-shocked, and 0.6 mm hydroperoxide-treated cultures, the means of three to six independent assays ±SEM are depicted. (B) Induction of hsp82-lacZ in response to moderate overexpression of α-Syn. EVS1013 cells, transformed with the plasmids indicated, were cultivated overnight in synthetic medium lacking uracil and containing 2% galactose and then processed as above (n = 8 for GST, n = 10 for WT, and n = 11 for the others). (C) Induction of hsp82-lacZ in α-Syn-expressing cells either subjected to an acute heat shock (red) or maintained at 30° (blue) (n = 3). The data presented in B and C represent replicate assays of the same transformants. Independent transformants gave essentially identical results.