TABLE 1.

List of strains constructed for use in dRbf synthetic lethal screen

Stock description	Abbreviation	Genotype
dRbf alleles	CAS-21	Su(p21)CAS-21/FM7c; +/CyO, P{p21-pGMR-33B}
SLS-15	w, Su(p21)SLS-15, P{ry[+t7.2] = neopFRT}18A/FM7c, ftz lacZ
Rescue construct	RbfR	y, w, P{dRbf-pExP-FRT2.1-3.5ey-FLP-5}/FM7c
Rescued dRbf   alleles	CAS-21+dRbR	w, Su(p21)CAS-21, PExp{FRT2.1[dRbf+, w+, 3.5ey-FLP]}; sp; e
SLS-15+dRbfR	w, Su(p21)S RbfSS2L LS-15, PExp{FRT2.1[dRbf+, w+, 3.5ey-FLP]}; sp; e
dRbf screening   stocks	RbfSS2L	w, Su(p21)SLS-15, PExp{FRT2.1[dRbf+, w+, 3.5ey-FLP]}; M(2)24F[1]P{w[+mC] = piM}36F   P{ry[+t7.2] = neoFRT}40A/CyO
RbfSS2R	w, Su(p21)SLS-15, PExp{FRT2.1[dRbf+, w+, 3.5ey-FLP]}; P{ry[+t7.2] = neoFRT}42D   P{w[+mC] = piM}45F M(2)53[1]/CyO
RbfSS3R	w, Su(p21)SLS-15, PExp{FRT2.1[dRbf+, w+, 3.5ey-FLP]}; P{ry[+t7.2] = neoFRT}82B   P{w[+mC] = piM}87E RpS3[*]/TM6B, Tb[1]
RbfSS3L	w, Su(p21)SLS-15, PExp{FRT2.1[dRbf+, w+, 3.5ey-FLP]}; Dp(1;3)sc[j4], y+, P{w+}, M(3)67C,   pi75c, P[ry+, hs-neo, FRT]80B; ry/TM6B
Male stocks	IsoFS2L	iso2 P{ry[+t7.2] = neoFRT}40A; P{ry[+7.2] = ey-FLP.N}6, ry[506]
IsoFS2R	P{ry[+t7.2] = neoFRT}42D iso2; P{ry[+7.2] = ey-FLP.N}6, ry[506]
IsoFS3L	P{ry[+7.2] = ey-FLP.N}5/CyO; iso3 P[ry+, hs-neo, FRT]80B/TM6B
IsoFS3R	P{ry[+7.2] = ey-FLP.N}5; P{ry[+t7.2] = neoFRT}82B iso3
dRbf+ MFRT   lines for   counterscreen	MFRT2L	w[*]; M(2)24F[1] P{w[+mC] = piM}36F P{ry[+t7.2] = neoFRT}40A/CyO
MFRT2R	w[*]; P{ry[+t7.2] = neoFRT}42D P{w[+mC] = piM}45F M(2)53[1]/CyO
MFRT3L	yw; Dp(1;3)sc[j4], y+, P{w+}, M(3)67C, pi75c, P[ry+, hs-neo, FRT]80B; ry/TM6B
MFRT3R	w[*]; P{ry[+t7.2] = neoFRT}82B P{w[+mC] = piM}87E RpS3[*]/TM6B, Tb[1]
ey-flp lines	EFL2	P{ry[+t7.2] = ey-FLP.N}6, ry[506]
EFL3	P{ry[+t7.2] = ey-FLP.N}5, ry[506]
CyO-GFP source	CyO-GFP	w; L[2] Pin[1]/CyO, P{w[+mC] = GAL4-Kr.C}DC3, P{w[+mC] = UAS− GFP.S65T}DC7
KE1 alleles	KE1-1	yw, FRT(2R)KE1-1/CyO
	KE1-2	yw, FRT(2R)KE1-2/CyO