TABLE 1.
Mutations detected at HP loci in the MMR-deficient MA lines
|
msh-2 MA lines
|
msh-6 MA lines
|
||||||
|---|---|---|---|---|---|---|---|
| Locus | HP length | +1 | −1 | Other | +1 | −1 | Other |
| (A:T) HPs | |||||||
| Y56A3A | 8 | 0 | 1 | 0 | 0 | 1 | 0 |
| C48B6 | 9 | 0 | 0 | 0 | 0 | 1 | 0 |
| M106 | 9 | 1 | 0 | 0 | 0 | 0 | 0 |
| B0284 | 10 | 0 | 1 | 0 | 0 | 1 | 0 |
| K07G5 | 10 | 0 | 0 | 0 | 0 | 1 | 0 |
| R10E4 | 11 | 0 | 2 | 0 | 0 | 4 | 0 |
| C48B6(a) | 12 | 0 | 3 | 0 | 0 | 3 | 0 |
| F02E9 | 15/13a | 0 | 12 | 0 | 1 | 2 | 0 |
| Totals: 1 | 19 | 0 | 1 | 13 | 0 | ||
| (G:C) HPs | |||||||
| Y73B6BL | 9/10b | 1 | 0 | 0 | 3 | 2 | 0 |
| F35D2 | 10 | 0 | 2 | 0 | 2 | 2 | 0 |
| C56E6 | 11 | 1 | 4 | 0 | 1 | 5 | (−2) |
| C01B12 | 13 | 3 | 4 | (−2) | 5 | 8 | 0 |
| Y113G7A | 14 | 2 | 14 | 0 | 6 | 9 | (+2, −2) |
| Y87G2A | 14 | 2 | 8 | 0 | 2 | 7 | 0 |
| Y48G9A | 16 | 3 | 6 | 0 | 4 | 5 | 0 |
| Totals: 12 | 38 | 1 | 23 | 38 | 3 | ||
Locus refers to the sequenced C. elegans cosmid or yeast artificial chromosome in which the mutation was found (PCR loci surveyed for mutation are named according to C. elegans cosmids, fosmids, and yeast artificial chromosomes; see supplementary Table 1 at http://www.genetics.org/supplemental/ for details). +1 refers to the number of single-base pairs insertions observed; −1 refers to the number of single-base pairs deletions observed. Other specific types of mutations are indicated in parentheses in the “Other” columns.
The msh-2 progenitor locus was 15 bp long whereas the msh-6 progenitor locus was 13 bp long.
The msh-2 progenitor locus was 9 bp long and the msh-6 progenitor locus was 10 bp long.