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. 2005 May;170(1):355–363. doi: 10.1534/genetics.104.039362

TABLE 1.

Recovery of G418R colonies following DSB induction

Cell linea Cells plated
(millions)b
No. of G418R
colonies
Colony
frequency (×104)c
MT4  5 1931 3.86
MT7  5 1152 2.30
MT19  5 1643 3.28
CB2  5 1530 3.06
CB6 15  285 0.19
CB9  5 4036 8.07
a

Cell lines designated “MT” were derived from MT+ cells that express functional Msh2; cell lines designated “CB” were derived from clone B cells that are Msh2 deficient.

b

Cells were electroporated with pSce and were plated into G418 selection two days post-transfection, as described in materials and methods.

c

Calculated as number of G418R colonies divided by number of cells plated into selection.