TABLE 1.
Cell linea | Cells plated (millions)b |
No. of G418R colonies |
Colony frequency (×104)c |
---|---|---|---|
MT4 | 5 | 1931 | 3.86 |
MT7 | 5 | 1152 | 2.30 |
MT19 | 5 | 1643 | 3.28 |
CB2 | 5 | 1530 | 3.06 |
CB6 | 15 | 285 | 0.19 |
CB9 | 5 | 4036 | 8.07 |
Cell lines designated “MT” were derived from MT+ cells that express functional Msh2; cell lines designated “CB” were derived from clone B cells that are Msh2 deficient.
Cells were electroporated with pSce and were plated into G418 selection two days post-transfection, as described in materials and methods.
Calculated as number of G418R colonies divided by number of cells plated into selection.