Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of a mutation blocking IMP biosynthesis pathway on accumulation of AICAR. Yeast strains transformed with a plasmid carrying the ADE1-lacZ fusion (P115) were grown for 6 hr in SD casa medium containing adenine at either low (0.025 mm low ade) or high concentration (0.3 mm, high ade). β-Galactosidase activity was then measured as described in materials and methods. Strains were BY4742 (wild type), Y14244 (ade8), Y1095 (ade16 ade17), Y1164 (ade8 ade16 ade17), and Y16591 (ade3).
