Figure 1.—

Oligonucleotide primers for RT-PCR amplification and 5′- and 3′-RACE of RH1, SWS1, and M/LWS opsin mRNAs. (A) All internal primers are expected to amplify the codon positions between 248 and 300 of all opsin cDNAs and the RH1-, SWS1-, and LWS-specific internal primers are expected to amplify the codon positions between 50–70 and 260–280. (B) The EcoRI and SalI sites are boxed in the forward and reverse primers, respectively, and were used for cloning the amplified products into the expression vector pMT5. A Kozak sequence (CCACC) was inserted between the EcoRI site and the initiation codon to promote translation.