TABLE 2.

Mutagenesis results and the effects of amino acid changes on λ_max-shift

Pigment	Amino acid change	λmax (nm)	Estimate (nm)
RH1	WT	496	ZRh = 498
N83D	498	θD83N = −2
SWS1	WT	419	ZB = 360
S86F	367	θF86S = 51
I93T	413	θT93I = 0
V116L	416	θL116V = −1
S86F/I93T	359	θF86S×T93I = 5
S86F/V116L	360	θF86S×L116V = 3
I93T/V116L	411	θT93I×L116V = 8
S86F/I93T/V116L	360	θF86S×T93I×L116V = −7
LWS	WT	552	ZR = 558
	A180S	558	θS180A = −6