TABLE 3.
Development of embryos produced byklp3A mutant females
| Developmental stage (%)
|
|||||||
|---|---|---|---|---|---|---|---|
| Maternal genotype | One nucleus |
Two nuclei |
Cycles 2–9 |
Cycles 10–13 |
Cellular blastoderm |
Later | N |
| klp3Amei-352 | 62.6 | 25.2 | 7.3 | 3.3 | 0 | 1.6 | 123 |
| klp3Amei-352/klp3A1124 | 67.4 | 4.7 | 10.2 | 12.6 | 1.9 | 3.3 | 215 |
| klp3Amei-352/klp3A521 | 89.4 | 9.7 | 1.0 | 0 | 0 | 0 | 207 |
| klp3Amei-352/klp3A835 | 85.7 | 5.9 | 2.1 | 4.2 | 2.1 | 0.8 | 238 |
| klp3A835 | 68.8 | 10.2 | 17.6 | 1.7 | 1.7 | 0 | 176 |
| klp3Amei-352/FM7w | 3.1 | 7.3 | 45.8 | 26.6 | 10.9 | 6.3 | 192 |
| w1118 | 3.8 | 5.9 | 44.6 | 30.1 | 12.4 | 3.2 | 186 |
Embryos at 0–2.5 hr were collected from females of the stated maternal genotypes on grape juice agar plates at 25° prior to fixation and immunofluorescence analysis (see materials and methods).