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. 2005 Aug;170(4):1611–1621. doi: 10.1534/genetics.104.031401

TABLE 3.

Cbs library statistics

Library Insert size
range
Average insert
size
% insert-positive
clonesa
% Cbs-positive
clonesb
% additive PCR
productsc
Sau3A 2.0–0.2 kb 0.9 kb 75 27 45
Sheared 2.0–0.2 kb 0.75 kb 79 44 46
a

Percentage of clones in which the CP1 adaptor primer amplified a PCR product in colony PCR screening.

b

Percentage of insert-positive clones that gave a positive hybridization signal on a dot blot probed with the GCbs oligo.

c

Percentage of Cbs-positive clones in which the length of the products primed with CP1 and CCbs and CP1 and GCbs added up to the full-length insert (MIC-C + MIC-G = MIC-FL; see Figure 1).