TABLE 3.
Library | Insert size range |
Average insert size |
% insert-positive clonesa |
% Cbs-positive clonesb |
% additive PCR productsc |
---|---|---|---|---|---|
Sau3A | 2.0–0.2 kb | 0.9 kb | 75 | 27 | 45 |
Sheared | 2.0–0.2 kb | 0.75 kb | 79 | 44 | 46 |
Percentage of clones in which the CP1 adaptor primer amplified a PCR product in colony PCR screening.
Percentage of insert-positive clones that gave a positive hybridization signal on a dot blot probed with the GCbs oligo.
Percentage of Cbs-positive clones in which the length of the products primed with CP1 and CCbs and CP1 and GCbs added up to the full-length insert (MIC-C + MIC-G = MIC-FL; see Figure 1).