Figure 5.—

Gel-filtration analysis of Gtr1p in wild-type and gtr1Δ strains. Gtr1p appears at the same location (void) with Rpc19p in addition to the smaller molecular weight fractions in the S300 gel-filtration analysis. Disruption of GTR1 leads to an accumulation of the smaller Rpc19p-containing complex. Accumulation of A135 and A190 in the same fraction as Rpc19p was not observed in the gtr1Δ strain. (Top) HA-tagged Gtr1p was detected with anti-HA antibody. Molecular mass is indicated above by arrows. Yeast strains are indicated at the left: gtr1Δ, NBW5gtr1Δ; gtr1Δ + HA-GTR1, NBW5 gtr1Δ expressing HA-tagged Gtr1p. This experiment was performed twice with similar results.