Table 2. Effect of various metabolites on AtADCS activity.
The Ki values were calculated from the equations developed by Segel [32] for a partial competitive inhibition. Results are means±S.E.M. for triplicate determinations.
| Metabolite | Ki (μM) |
|---|---|
| Aromatic amino acids (phenylalanine, tyrosine, tryptophan) | No significant effect |
| pABA | No significant effect |
| Tetrahydrofolate derivatives (H4PteGlu1, 5-CH3-H4PteGlu1, 5-CHO-H4PteGlu1) | No significant effect |
| Folic acid (monoglutamate) | 3000±500 |
| Dihydropterin | 30±10 |
| Dihydropteroate | 15±5 |
| H2PteGlu1 | 10±3 |
| H2PteGlu5 | 8±3 |
| MTX | 1±0.3 |