Figure 5. NRG2β inhibits EGF binding to the EGFR-S442F mutant more potently than it inhibits EGF binding to the EGFR-Wt.

32D cells that express the EGFR-Wt or the EGFR-S442F mutant were incubated with increasing concentrations of NRG2β and 1 nM ¹²⁵I-EGF. The net binding of ¹²⁵I-EGF to EGFR was determined as described above. The graph shown is representative of results obtained from three independent experiments.