FIGURE 4:

Effect of DNA sequence context at adducts on the incision efficiency of UvrABC. The 5′-terminally labeled DNA substrates adducted with AF and AAF in TG^*T and CG^*C sequences were incubated with UvrABC (UvrA, 15 nM; UvrB, 250 nM; and UvrC, 100 nM) in the UvrABC buffer at 37 °C for the indicated periods. The incision products were analyzed on a 12% polyacrylamide urea denatureing gel. Incision efficiency or rate was determined for each of the substrates as the slope of a linear regression line of the kinetic data.