FIGURE 5:

Binding of UvrA protein to the AAF-DNA substrates in TG^*T and CG^*C sequences. The 5′-terminally labeled DNA substrates were incubated with varying concentrations of UvrA at 37 °C for 15 min in the UvrABC buffer without ATP. The binding products were analyzed on a 3.5% native polyacrylamide gel. Free DNA represents the unbound DNA substrates, and the UvrA-DNA represents the protein-DNA complex formation.