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. Author manuscript; available in PMC: 2006 Apr 28.
Published in final edited form as: Biochemistry. 2005 Jan 25;44(3):971–978. doi: 10.1021/bi048208a

Table 1:

Proteolysis Fragments of hRPA Containing ModifiedLysinesa

fragment modified K protection
RPA70
82-91 88 -
158-167 163 -
164-183 167 -
168-183 183 +
202-210 206 -
217-234 220 -
235-253 244 -
254-267 263 +
260-267 259 +
314-331 324 -
325-335 331 -
340-344 343 +
489-499 489 +
500-511 502 -
576-586 577 +
587-600 588 +
589-600 595 -
RPA32
38-40 38 -
82-105 93 -
128-139 138 -
139-145 139 -
RPA14
31-39 33 -
40-72 49 -
a

Tryptic digest fragments of hRPA subunits p70, p32, and p14 are shown with the modified lysine residue indicated.Lysines that are shown to be protected from modification in the presence of ssDNA are indicated by (+) while residues readily modified in the presence and absence of ssDNA are indicated by (-).Lysine residues that make direct contact with ssDNA are shown in bold.