Table 1.

Diffusion coefficients, inferred radii of gyration, and topology-dependent diffusion ratios and radius of gyration ratios for linear, relaxed circular, and supercoiled DNA molecules

DNA construct	Size, kbp	Length,* μm	DL, μm2/s	RG,L, μm	DC, μm2/s	RG,C, μm	Ds,† μm2/s	C = DC/DL	G = RG,L/RG,C
pYES2‡	5.9	2.65	1.28	0.213
pPIC9K〈TRL5〉	11.1	4.99	0.976	0.279	1.31	0.173	1.65	1.35	1.61
pCC1FOSTM45	45	20.25	0.437	0.624	0.554	0.410	0.598	1.27	1.52
CTD-2342K16	112.8	50.81	0.260	1.05	0.35	0.645	0.490	1.36	1.63
CTD-2609C22	183.5	82.66	0.197	1.38	0.26	0.867	0.334	1.33	1.60
CTD-2657L24	287.1	129.32	0.139	1.96	0.180	1.26	0.226	1.30	1.56

*Accounting for the increase in length caused by YOYO-I, as described in ref. 56.

^†Samples had ≈50–80% supercoiled molecules, as described in the text.

^‡Relaxed circular and supercoiled pYES2 moved too quickly to accurately track with our current setup.