Fig. 1.
N-terminal HA-tagged hCTR1N15Q construct is functional in growth complementation under copper starvation. S. cerevisiae yeast deficient in high-affinity copper uptake (Δctr1,3) were transformed with the HA-tagged hCTR1 construct used in this study (labeled as HAhCTR1N15Q) or vector only (vector). (Upper) Copper starvation was achieved with plates that contained the copper chelator bathocuproinedisulfonic acid (BCS) and no additional copper. (Lower) Shown is control growth on plates containing excess copper sulfate (50 μM) without any chelator.