Table 1.
Kinetics of PTEN membrane binding in living cells
| Cells (temperature, °C) | PTEN allele | Dissociation rate, ms | Diffusion coefficient, μm2/s |
|---|---|---|---|
| D. discoideum(22) | DdPTEN-YFP | 310 (n = 2,040; 6 cells) | 0.15 ± 0.13 (n = 230; 4 cells) |
| PTEN-YFP | 330 (n = 875; 12 cells) | 0.18 ± 0.15 (n = 44; 4 cells) | |
| HEK293 (22) | PTEN-YFP | 180 (n = 828; 12 cells) | 0.18 ± 0.19 (n = 165; 12 cells) |
| PTEN (C124S;A4)-YFP | 290 (n = 657; 4 cells) | 0.34 ± 0.21 (n = 194; 4 cells) | |
| HEK293 (33) | PTEN-YFP | 150 (n = 270; 5 cells) | 0.41 ± 0.60 (n = 138; 5 cells) |
| PTEN (C124S;A4)-YFP | 130 (n = 805, 4 cells) | 0.41 ± 0.39 (n = 107; 4 cells) |