Fig. 5.

The expression of Mesp2 is not achieved solely by RBPJκ-dependent Notch signaling. (A) Transgenic analyses reveal that somite-specific reporter expression can still be observed by using the P2EΔAmC construct, which contains a deletion of site A and mutations in site C. The numbers of β-gal-positive embryos are indicated for each image (β-gal-positive/transgene-positive). (B) The expression of a dominant-negative RBPJκ diminishes reporter activation by Tbx6 for both the wild-type (wt) and P2EΔAmC (Tbx6+R218H, purple bars) vectors. Wild-type RBPJκ also strongly suppressed reporter activity driven by Tbx6 (Tbx6+RBPJκ, orange bars). Error bars represent the standard deviation in six independent experiments.