Fig. 2.

Receptor editing at the light chain locus. (A) Allelic inclusion revealed by the expression of the hCκ chain in B220⁺ splenocytes from H^121/+L^121/h mice; populations “G,” “D,” and “E” (abbreviations stand for “GPI only,” “Dual,” and “Eliminated”) are defined in relation to their GPI-binding capacity (Middle). (B) Isotypic inclusion and the usage of λ L chains in B220⁺ splenocytes of adult H^121/+L^121/+ mice and control littermates. (C) RS-IRS-1 excision (a 1.1-kb band) was determined with 3-fold serial dilutions of genomic DNA from sorted cells of the populations “G,” “D,” and “E” (starting with amount equivalent to 5,000 cells) using flanking PCR primers (small arrowheads). (D) B220⁺ splenocytes of H^121/+L^121/+Rag^+/− and H^121/+L^121/+Rag^−/− mice. Representative of three to five mice.